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KMID : 0363219920300030317
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Korean Journal of Dermatology
1992 Volume.30 No. 3 p.317 ~ p.324
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Expression of Type IV Collagen and Laminin Genes by Skin Fibroblasts in Culture
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Abstract
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Basement membrane zone gene expression by fibroblast cultures was examined by molecular hybridizations with human sequence specific cDNAs corresponding to type IV procollagen and laminin subunit polypeptides. Northern transfer analysis with total
RNA
revealed the presence of specific mRNA transcripts for α1 (IV) and α2 (IV) chains of type IV procollagen as well as B1 and B2 chains of laminin. Laminin A chain mRNAs were not detected using the same RNA preparations. The molecular size of α1
(IV)
and α2 (IV) procollagen mRNA revealed 6.8kb and 6.7kb, respectively. The molecular size of B1 chain of laminin revealed 5.6kb, and B2 chain revealed 8.2 and 5.5kb polymorphic transcripts. In slotblot analysis using densitometer, steady-state
levels of
type IV procollagen and laminin mRNAs indicated that they were in relatively low abundance, as compaired with type 1 procollagen mRNA. Quantitative levels of α1 (IV) and laminin B1 chain mRNAs were more abundant than those of α2 (IV) and
laminin
B2
mRNAs. The mRNA ratio of α1 (IV)/α2 (IV) and laminin B1/B2 were 1.9 and 1.5, respectively. These results demonstrate evidence for differential regulation of the expression of different basement membrane zone molecules during the formation of
basement
membrane. (Kor J Dermatol 1992:30(3) ;317-324)
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